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fluorescent nadh biosensor peredox mcherry  (Addgene inc)


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    Addgene inc fluorescent nadh biosensor peredox mcherry
    Fluorescent Nadh Biosensor Peredox Mcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescent nadh biosensor peredox mcherry/product/Addgene inc
    Average 93 stars, based on 24 article reviews
    fluorescent nadh biosensor peredox mcherry - by Bioz Stars, 2026-06
    93/100 stars

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    nad nadh redox states fluorescent biosensor  (Addgene inc)
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    a Nuclear SIRTUIN (SIRT1, SIRT6, SIRT7) activity of aged mice brain with or without nicotine at 13 months of age ( n = 3, biologically independent samples/group). b Nicotine has no district effects on NAMPT-SIRT6/ SIRT7 interaction. c The total <t>NAD</t> + levels of SIRT1 knockdown cells after nicotine treatment ( n = 3, biologically independent samples/group). d Nicotine (10 ng/mL) has no district enhancement on SIRT1 interact with other substrates (p53, PGC-1, NF-κB). e The NAMPT inhibitor FK866 abolished the effect of nicotine ( n = 3, biologically independent samples/group). f SIRT1 inhibitor has no district effect on SIRT1-NAMPT interaction. g The effect of SIRT1 activator SRT1720 (2.5 μg/mL) and inhibitor Selisistat (5 μmol/L) on SIRT1-NAMPT interaction. h Cotinine has no effect on SIRT1-NAMPT interaction. All western blotting images and graphs are representative of three independent experiments. Data are means ± SEM. p values were determined by two-sided Student’s t test ( a ), or one-way ANOVA with Tukey’s multiple comparisons tests ( c , e ).
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    a Nuclear SIRTUIN (SIRT1, SIRT6, SIRT7) activity of aged mice brain with or without nicotine at 13 months of age ( n = 3, biologically independent samples/group). b Nicotine has no district effects on NAMPT-SIRT6/ SIRT7 interaction. c The total NAD + levels of SIRT1 knockdown cells after nicotine treatment ( n = 3, biologically independent samples/group). d Nicotine (10 ng/mL) has no district enhancement on SIRT1 interact with other substrates (p53, PGC-1, NF-κB). e The NAMPT inhibitor FK866 abolished the effect of nicotine ( n = 3, biologically independent samples/group). f SIRT1 inhibitor has no district effect on SIRT1-NAMPT interaction. g The effect of SIRT1 activator SRT1720 (2.5 μg/mL) and inhibitor Selisistat (5 μmol/L) on SIRT1-NAMPT interaction. h Cotinine has no effect on SIRT1-NAMPT interaction. All western blotting images and graphs are representative of three independent experiments. Data are means ± SEM. p values were determined by two-sided Student’s t test ( a ), or one-way ANOVA with Tukey’s multiple comparisons tests ( c , e ).

    Journal: Nature Communications

    Article Title: Nicotine rebalances NAD + homeostasis and improves aging-related symptoms in male mice by enhancing NAMPT activity

    doi: 10.1038/s41467-023-36543-8

    Figure Lengend Snippet: a Nuclear SIRTUIN (SIRT1, SIRT6, SIRT7) activity of aged mice brain with or without nicotine at 13 months of age ( n = 3, biologically independent samples/group). b Nicotine has no district effects on NAMPT-SIRT6/ SIRT7 interaction. c The total NAD + levels of SIRT1 knockdown cells after nicotine treatment ( n = 3, biologically independent samples/group). d Nicotine (10 ng/mL) has no district enhancement on SIRT1 interact with other substrates (p53, PGC-1, NF-κB). e The NAMPT inhibitor FK866 abolished the effect of nicotine ( n = 3, biologically independent samples/group). f SIRT1 inhibitor has no district effect on SIRT1-NAMPT interaction. g The effect of SIRT1 activator SRT1720 (2.5 μg/mL) and inhibitor Selisistat (5 μmol/L) on SIRT1-NAMPT interaction. h Cotinine has no effect on SIRT1-NAMPT interaction. All western blotting images and graphs are representative of three independent experiments. Data are means ± SEM. p values were determined by two-sided Student’s t test ( a ), or one-way ANOVA with Tukey’s multiple comparisons tests ( c , e ).

    Article Snippet: HT22 cells were transfected with NAD + -NADH redox states fluorescent biosensor : pcDNA3.1-Peredox-mCherry (Addgene: #32383) and pcDNA3.1-Peredox-mCherry-NLS (Addgene: #32384).

    Techniques: Activity Assay, Knockdown, Western Blot